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Biblioteca (s) : |
INIA La Estanzuela. |
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Fecha : |
21/02/2014 |
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Actualizado : |
01/10/2019 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Autor : |
COZZOLINO, D.; DELUCCHI, M.I.; KHOLI, M.; VÁZQUEZ, D. |
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Afiliación : |
DANIEL COZZOLINO GÓMEZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARIA INES DELUCCHI ZAPARRART, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MOHAM KHOLI, MOHAM, International Center for Wheat and Maize Improvement (CIMMYT).; DANIEL VÁZQUEZ PEYRONEL, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
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Título : |
Use of near infrared reflectance spectroscopy to evaluate quality characteristics in whole-wheat grain. [Uso de la espectroscopía de reflectancia en el infrarrojo cercano para evaluar características de calidad en trigo]. |
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Fecha de publicación : |
2006 |
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Fuente / Imprenta : |
Agricultura Técnica, December 2006, Volume 66, Issue 4, Pages 370-375. |
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DOI : |
10.4067/S0365-28072006000400005 |
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Idioma : |
Inglés |
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Notas : |
Article history:Recibido: 17 de octubre de 2005/Aprobado: 30 de marzo de 2006. |
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Contenido : |
ABSTRACT:
The aim of this work was to explore the potential of visible (Vis) and near infrared reflectance (NIR) spectroscopy to measure quality characteristics in whole grain wheat (Triticum aestivum L.) as a tool in breeding programs. A total of 100 samples were analyzed by the reference methods for crude protein (CP), wet gluten (WG) and sodium dodecyl sulfate (SDS) sedimentation test. Whole grain samples were scanned in a NIR monochromator instrument (400-2500 nm) in reflectance. Partial least squares (PLS) were used to develop calibration equations for the quality characteristics in whole wheat. Calibration models were validated using an independent set of samples (n = 50) randomly selected from the population set. The uncertainty of the PLS models was evaluated by the standard error of prediction (SEP). The SEP obtained were 0.35% for CP, 2.04 for SDS and 4.14% for WG. It was concluded that NIR spectroscopy might be used as a screening tool to segregate early generations of wheat genotypes. At a later stage is needed to improve the accuracy of the NIR calibrations, broadening the calibration spectra with the incorporation of more genotypes and different crop years.
RESUMEN:
El objetivo de este trabajo fue explorar el potencial de la espectroscopía en el visible (Vis) e infrarrojo cercano (NIR) para medir características de calidad en el trigo (Triticum aestivum L.) para su uso en programas de mejoramiento. Cien muestras fueron analizadas por el método de referencia para proteína cruda (CP), gluten húmedo (WG) y sulfato de dodecil de sodio (SDS) o prueba de sedimentación. Las muestras de trigo se analizaron en un instrumento NIR (400-2500 nm) en reflectancia. El método de los cuadrados mínimos parciales (PLS) fue utilizado para desarrollar las ecuaciones de calibración para las características de calidad en trigo. Los modelos de calibración se validaron utilizando un conjunto independiente de muestras (n = 50) aleatoriamente escogido del conjunto de la población. La incertidumbre de los modelos PLS de calibración fue evaluada usando el error estándar de la predicción (SEP). El SEP obtenido fue de 0,35% para CP, 2,04 para SDS y 4,14% para WG. Se concluyó que la espectroscopía de NIR podría utilizarse como una herramienta de selección para segregar genotipos de trigo en generaciones tempranas. En una etapa posterior se necesita mejorar la precisión de los análisis NIR, ampliando el espectro de calibración con la incorporación de más genotipos y diferentes años de cultivo. MenosABSTRACT:
The aim of this work was to explore the potential of visible (Vis) and near infrared reflectance (NIR) spectroscopy to measure quality characteristics in whole grain wheat (Triticum aestivum L.) as a tool in breeding programs. A total of 100 samples were analyzed by the reference methods for crude protein (CP), wet gluten (WG) and sodium dodecyl sulfate (SDS) sedimentation test. Whole grain samples were scanned in a NIR monochromator instrument (400-2500 nm) in reflectance. Partial least squares (PLS) were used to develop calibration equations for the quality characteristics in whole wheat. Calibration models were validated using an independent set of samples (n = 50) randomly selected from the population set. The uncertainty of the PLS models was evaluated by the standard error of prediction (SEP). The SEP obtained were 0.35% for CP, 2.04 for SDS and 4.14% for WG. It was concluded that NIR spectroscopy might be used as a screening tool to segregate early generations of wheat genotypes. At a later stage is needed to improve the accuracy of the NIR calibrations, broadening the calibration spectra with the incorporation of more genotypes and different crop years.
RESUMEN:
El objetivo de este trabajo fue explorar el potencial de la espectroscopía en el visible (Vis) e infrarrojo cercano (NIR) para medir características de calidad en el trigo (Triticum aestivum L.) para su uso en programas de mejoramiento. Cien muestras fueron analizadas por el método de refer... Presentar Todo |
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Palabras claves : |
GLUTEN HÚMEDO; GRAIN QUALITY; GRANO DE TRIGO; PROTEIN; SDS; WET GLUTEN; WHOLE WHEAT. |
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Thesagro : |
NIRS; PROTEÍNA; TRIGO; TRITICUM AESTIVUM. |
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Asunto categoría : |
F01 Cultivo |
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URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/13383/1/Uso-de-la-espectroscopia-de-reflectancia-en-el-inf.pdf
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Marc : |
LEADER 03624naa a2200313 a 4500
001 1034984
005 2019-10-01
008 2006 bl uuuu u00u1 u #d
024 7 $a10.4067/S0365-28072006000400005$2DOI
100 1 $aCOZZOLINO, D.
245 $aUse of near infrared reflectance spectroscopy to evaluate quality characteristics in whole-wheat grain. [Uso de la espectroscopía de reflectancia en el infrarrojo cercano para evaluar características de calidad en trigo].$h[electronic resource]
260 $c2006
500 $aArticle history:Recibido: 17 de octubre de 2005/Aprobado: 30 de marzo de 2006.
520 $aABSTRACT: The aim of this work was to explore the potential of visible (Vis) and near infrared reflectance (NIR) spectroscopy to measure quality characteristics in whole grain wheat (Triticum aestivum L.) as a tool in breeding programs. A total of 100 samples were analyzed by the reference methods for crude protein (CP), wet gluten (WG) and sodium dodecyl sulfate (SDS) sedimentation test. Whole grain samples were scanned in a NIR monochromator instrument (400-2500 nm) in reflectance. Partial least squares (PLS) were used to develop calibration equations for the quality characteristics in whole wheat. Calibration models were validated using an independent set of samples (n = 50) randomly selected from the population set. The uncertainty of the PLS models was evaluated by the standard error of prediction (SEP). The SEP obtained were 0.35% for CP, 2.04 for SDS and 4.14% for WG. It was concluded that NIR spectroscopy might be used as a screening tool to segregate early generations of wheat genotypes. At a later stage is needed to improve the accuracy of the NIR calibrations, broadening the calibration spectra with the incorporation of more genotypes and different crop years. RESUMEN: El objetivo de este trabajo fue explorar el potencial de la espectroscopía en el visible (Vis) e infrarrojo cercano (NIR) para medir características de calidad en el trigo (Triticum aestivum L.) para su uso en programas de mejoramiento. Cien muestras fueron analizadas por el método de referencia para proteína cruda (CP), gluten húmedo (WG) y sulfato de dodecil de sodio (SDS) o prueba de sedimentación. Las muestras de trigo se analizaron en un instrumento NIR (400-2500 nm) en reflectancia. El método de los cuadrados mínimos parciales (PLS) fue utilizado para desarrollar las ecuaciones de calibración para las características de calidad en trigo. Los modelos de calibración se validaron utilizando un conjunto independiente de muestras (n = 50) aleatoriamente escogido del conjunto de la población. La incertidumbre de los modelos PLS de calibración fue evaluada usando el error estándar de la predicción (SEP). El SEP obtenido fue de 0,35% para CP, 2,04 para SDS y 4,14% para WG. Se concluyó que la espectroscopía de NIR podría utilizarse como una herramienta de selección para segregar genotipos de trigo en generaciones tempranas. En una etapa posterior se necesita mejorar la precisión de los análisis NIR, ampliando el espectro de calibración con la incorporación de más genotipos y diferentes años de cultivo.
650 $aNIRS
650 $aPROTEÍNA
650 $aTRIGO
650 $aTRITICUM AESTIVUM
653 $aGLUTEN HÚMEDO
653 $aGRAIN QUALITY
653 $aGRANO DE TRIGO
653 $aPROTEIN
653 $aSDS
653 $aWET GLUTEN
653 $aWHOLE WHEAT
700 1 $aDELUCCHI, M.I.
700 1 $aKHOLI, M.
700 1 $aVÁZQUEZ, D.
773 $tAgricultura Técnica, December 2006, Volume 66, Issue 4, Pages 370-375.
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Registro original : |
INIA La Estanzuela (LE) |
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Registro completo
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Biblioteca (s) : |
INIA Las Brujas. |
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Fecha actual : |
01/09/2020 |
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Actualizado : |
01/09/2020 |
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Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
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Circulación / Nivel : |
Internacional - -- |
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Autor : |
BERGAMO, L.W.; SILVA-BRANDÃO, K.L.; VICENTINI, R.; FRESIA, P.; AZEREDO-ESPIN, A.M.L |
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Afiliación : |
LUANA WALRAVENS BERGAMO, Universidade Estadual de Campinas (CBMEG-UNICAMP), Campinas SP, Brazil; KARINA LUCAS SILVA-BRANDÃO, Universidade Estadual de Campinas (CBMEG-UNICAMP), Campinas SP, Brazil; Universidade Federal do ABC (CCNH-UFABC), Santo André SP, Brazil; RENATO VICENTINI, Universidade Estadual de Campinas (UNICAMP), Campinas SP, Brazil; PABLO FRESIA, Unidad Mixta UMPI, Institut Pasteur Montevideo + INIA, Montevideo, Uruguay; ANA MARIA LIMA AZEREDO-ESPIN, Universidade Estadual de Campinas (UNICAMP), Campinas SP, Brazil; Universidade Estadual de Campinas (CBMEG-UNICAMP), Campinas SP, Brazil. |
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Título : |
Genetic differentiation of a new world screwworm fly population from uruguay detected by snps, mitochondrial dna and microsatellites in two consecutive years. |
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Fecha de publicación : |
2020 |
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Fuente / Imprenta : |
Insects, August 2020, Volume 11, Issue 8, Pages 1-16, Article number 539. OPEN ACCESS. DOI: https://doi.org/10.3390/insects11080539 |
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DOI : |
10.3390/insects11080539 |
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Idioma : |
Inglés |
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Notas : |
Article history: Received 9 July 2020; Revised 2 August 2020; Accepted 9 August 2020; Published 16 August 2020.
This article belongs to the Special Issue Population Genetics of Insects - https://www.mdpi.com/journal/insects/special_issues/population_genetics |
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Contenido : |
ABSTRACT.
The New World screwworm (NWS) fly, Cochliomyia hominivorax (Diptera: Calliphoridae), is an economically important ectoparasite currently distributed in South America and in the Caribbean basin. The successful eradication of this species in USA, Mexico and continental Central America was achieved by a control program based on the sterile insect technique (SIT). In order to implement a genetic control strategy over the NWS fly?s current area of occurrence, first, it is necessary to understand the species dynamics and population structure. In order to address this objective, the spatial genetic structure of the NWS fly was previously reported in South America based on different genetic markers; however, to date, no study has investigated temporal changes in the genetic composition of its populations. In the current study, the temporal genetic structure of a NWS fly population from Uruguay was investigated through two consecutive samplings from the same locality over an interval of approximately 18 generations. The genetic structure was accessed with neutral and under selection SNPs obtained with genotyping-by-sequencing. The results gathered with these data were compared to estimates achieved with mitochondrial DNA sequences and eight microsatellite markers. Temporal changes in the genetic composition were revealed by all three molecular markers, which may be attributed to seasonal changes in the NWS fly?s southern distribution. SNPs were employed for the first time for estimating the genetic structure in a NWS fly population; these results provide new clues and perspectives on its population genetic structure. This approach could have significant implications for the planning and implementation of management programs.
© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). MenosABSTRACT.
The New World screwworm (NWS) fly, Cochliomyia hominivorax (Diptera: Calliphoridae), is an economically important ectoparasite currently distributed in South America and in the Caribbean basin. The successful eradication of this species in USA, Mexico and continental Central America was achieved by a control program based on the sterile insect technique (SIT). In order to implement a genetic control strategy over the NWS fly?s current area of occurrence, first, it is necessary to understand the species dynamics and population structure. In order to address this objective, the spatial genetic structure of the NWS fly was previously reported in South America based on different genetic markers; however, to date, no study has investigated temporal changes in the genetic composition of its populations. In the current study, the temporal genetic structure of a NWS fly population from Uruguay was investigated through two consecutive samplings from the same locality over an interval of approximately 18 generations. The genetic structure was accessed with neutral and under selection SNPs obtained with genotyping-by-sequencing. The results gathered with these data were compared to estimates achieved with mitochondrial DNA sequences and eight microsatellite markers. Temporal changes in the genetic composition were revealed by all three molecular markers, which may be attributed to seasonal changes in the NWS fly?s southern distribution. SNPs were employed for the first time f... Presentar Todo |
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Palabras claves : |
Effective population size (Ne); Genotyping-by-sequencing (GBS); Insecticide resistance; Population; Population genomics. |
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Asunto categoría : |
A50 Investigación agraria |
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URL : |
https://www.mdpi.com/2075-4450/11/8/539/htm
https://www.mdpi.com/2075-4450/11/8/539/pdf
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Marc : |
LEADER 03179naa a2200253 a 4500
001 1061289
005 2020-09-01
008 2020 bl uuuu u00u1 u #d
024 7 $a10.3390/insects11080539$2DOI
100 1 $aBERGAMO, L.W.
245 $aGenetic differentiation of a new world screwworm fly population from uruguay detected by snps, mitochondrial dna and microsatellites in two consecutive years.$h[electronic resource]
260 $c2020
500 $aArticle history: Received 9 July 2020; Revised 2 August 2020; Accepted 9 August 2020; Published 16 August 2020. This article belongs to the Special Issue Population Genetics of Insects - https://www.mdpi.com/journal/insects/special_issues/population_genetics
520 $aABSTRACT. The New World screwworm (NWS) fly, Cochliomyia hominivorax (Diptera: Calliphoridae), is an economically important ectoparasite currently distributed in South America and in the Caribbean basin. The successful eradication of this species in USA, Mexico and continental Central America was achieved by a control program based on the sterile insect technique (SIT). In order to implement a genetic control strategy over the NWS fly?s current area of occurrence, first, it is necessary to understand the species dynamics and population structure. In order to address this objective, the spatial genetic structure of the NWS fly was previously reported in South America based on different genetic markers; however, to date, no study has investigated temporal changes in the genetic composition of its populations. In the current study, the temporal genetic structure of a NWS fly population from Uruguay was investigated through two consecutive samplings from the same locality over an interval of approximately 18 generations. The genetic structure was accessed with neutral and under selection SNPs obtained with genotyping-by-sequencing. The results gathered with these data were compared to estimates achieved with mitochondrial DNA sequences and eight microsatellite markers. Temporal changes in the genetic composition were revealed by all three molecular markers, which may be attributed to seasonal changes in the NWS fly?s southern distribution. SNPs were employed for the first time for estimating the genetic structure in a NWS fly population; these results provide new clues and perspectives on its population genetic structure. This approach could have significant implications for the planning and implementation of management programs. © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
653 $aEffective population size (Ne)
653 $aGenotyping-by-sequencing (GBS)
653 $aInsecticide resistance
653 $aPopulation
653 $aPopulation genomics
700 1 $aSILVA-BRANDÃO, K.L.
700 1 $aVICENTINI, R.
700 1 $aFRESIA, P.
700 1 $aAZEREDO-ESPIN, A.M.L
773 $tInsects, August 2020, Volume 11, Issue 8, Pages 1-16, Article number 539. OPEN ACCESS. DOI: https://doi.org/10.3390/insects11080539
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